Designing Effective Recombinant Molecules

By Icy D’Silva.

Published by The Design Collection

Format Price
Article: Print $US10.00
Article: Electronic $US5.00

Recombinant technology which involves the incorporation of genetic material from one organism to another using laboratory tools and bioinformatics, has revolutionized the engineering of molecules for a variety of purposes, including therapeutics. Improved stability, reduced allergenicity, and clinical efficacy are some of the desired characteristics of recombinant molecules. Designing effective recombinant molecules requires extensive knowledge of the native molecule, its physical and chemical properties, and its structure-function relationship, as well as the molecular traits of the biology, genetics or environment that are critical for the identification and selection of suitable targets for the recombinant molecule. Strategically designed effective recombinant molecules are a genuine prospect for the socio-economic welfare of humanity and for a safe environment. Strategies for designing effective recombinant molecules may be based on a number of principles, elements, factors, and methods, including rational design, DNA shuffling, fusion of molecules, directed evolution, phage display, ribosome display, site-specific post-translational modification, and site-directed mutagenesis. As a research study, recombinant ovalbumin molecules that were strategically designed to protect mice against egg allergy, were effective in mice as demonstrated by in vivo monitoring and in vitro assays, and may hold promise as potential immunotherapeutic agents against egg allergy in humans. Strategically designed recombinant ovalbumin may also serve as a safe substitute for native allergenic ovalbumin in food products. This theoretical discourse will describe the advances in and strategies for designing effective recombinant molecules.

Keywords: Designing Recombinant Molecules, Recombinant Technology

Design Principles and Practices: An International Journal, Volume 4, Issue 6, pp.65-72. Article: Print (Spiral Bound). Article: Electronic (PDF File; 584.653KB).

Icy D’Silva

Graduate Student, Department of Food Science, Ontario Agricultural College, University of Guelph, Guelph, Ontario, Canada

Icy D’Silva (Ida Iris Icy D’Silva) earned her Master of Science degree studying ‘Anti-Lipopolysaccharide Antibody-Mediated Disease Resistance against Pseudomonas aeruginosa O6ad in Tobacco’ with Professor J. Christopher Hall as her Mentor and Advisor, from the University of Guelph (Canada) renowned for its excellence in teaching, research and innovation. Icy is presently a Doctor of Philosophy Graduating Student at the University of Guelph having been involved in the study of recombinant molecules towards resistance against egg allergy with Professor Yoshinori Mine as Advisor and Professor J. Christopher Hall as her Mentor. Her expertise in biology has been widely chronicled and she has contributed to a number of academic journals, articles and book chapters. Icy has been enthusiastically participating in innumerable opportunities offered by the University of Guelph. Icy has been a University of Guelph - Ontario Agricultural College - Graduate Student Senator, and a University of Guelph - Food Science Department - Graduate Students’ Representative. Icy is equally active in serving the Guelph and Canadian community in particular, as well as the World community at large. Icy is an avid reader and writer.

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